Analysis Note

ControlHuman Lymphocytes

Application

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

This Milli-Mark Anti-CD42b-PE Antibody, clone AN51 is validated for use in FC for the detection of CD42b.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD42b is a 145 kDa protein, which together with CD42c forma a 160 kDa heterodimer composed of an alpha-chain and a beta-chain, respectively. The subunits are linked together by a disulphide bond. CD42b and CD42c forms a non-covalent complex together with CD42a and CD42d in the platelet plasma membrane. The CD42 complex serves as a receptor for von Willebrand factor and thrombin and mediates adhesion of platelets to subendothelial matrices (exposed upon damage to the endothelium) at high shear rates. The binding sites for von Willebrand factor and thrombin lies on CD42b (de Haas, 2002). CD42 is expressed on megakarytocytes and platelets (McMichael, 1981).

Immunogen

Mixture of human platelets and lymphocytes.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

Purified Mouse monoclonal IgG2a conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Quality

Evaluated by flow cytometry using human lymphocytes

Specificity

Antibody recognizes Human CD42b antigen.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

145 kDa (de Haas, 2002)