Analysis Note
ControlHuman Lymphocytes
Application
Research Sub CategoryImmunoglobulins & Immunology
Research CategoryInflammation & Immunology
This Milli-Mark Anti-CD42b-PE Antibody, clone AN51 is validated for use in FC for the detection of CD42b.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
CD42b is a 145 kDa protein, which together with CD42c forma a 160 kDa heterodimer composed of an alpha-chain and a beta-chain, respectively. The subunits are linked together by a disulphide bond. CD42b and CD42c forms a non-covalent complex together with CD42a and CD42d in the platelet plasma membrane. The CD42 complex serves as a receptor for von Willebrand factor and thrombin and mediates adhesion of platelets to subendothelial matrices (exposed upon damage to the endothelium) at high shear rates. The binding sites for von Willebrand factor and thrombin lies on CD42b (de Haas, 2002). CD42 is expressed on megakarytocytes and platelets (McMichael, 1981).
Immunogen
Mixture of human platelets and lymphocytes.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form
Protein A purified
Purified Mouse monoclonal IgG2a conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Quality
Evaluated by flow cytometry using human lymphocytes
Specificity
Antibody recognizes Human CD42b antigen.
Storage and Stability
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Target description
145 kDa (de Haas, 2002)
This product has met the following criteria to qualify for the following awards: